Areas of Interest
Areas of Interest
Cell and Gene Therapy (CGT)
Our high-throughput, automated workflow on OptoBot® 1000 or its series products isolates target single cells from transient co-cultures for downstream analysis, enabling multidimensional, single-cell resolution characterization of T cell potency. Images captured during isolation allow for the tracking of specific cells or cell types. Ideal for assays involving the co-culture of multiple cell types, our platform correlates on-chip phenotypic data with single-cell RNA sequencing (scRNA-seq) results. Our unique approach connects single-cell functional assessments with genomic data, providing integrated insights into the biology of individual T cells.
Cell and Gene Therapy (CGT)

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T Cell Profiling Kit

Uncover Hidden Depths of T Cell Function with the OptoBot® T Cell Profiling Kit. The platform can evaluate the function of individual T cells from multiple dimensions, including cytokine secretion, cell surface markers, and dynamic killing of target cells. It outputs precise functional kinetic parameters and time-series data, enabling a direct correlation between a cell's "functional phenotype" and its "genotype/transcriptome". This reveals complex relationships between functional outputs that cannot be obtained with traditional methods.

Basic Universal Kit

Provide an optimal experimental environment for your workflow.

OptoBot ®1000 Universal Kit

Available Items for Your Kit

To perform a T Cell Profiling assay, you will need to select chips and beads, and, if performing downstream sequencing analysis, a sequencing kit.

1. Chip Type

Chip type needed for T cell profiling assay.

OptoTrap Chip® 3500

~3,500 NanoPen chambers per chip​

2. Bead Type

Conjugate beads with an antigen or peptide for enrichment or co-culture.

Antigen Presenting Bead Kit, HLA-A*11:01

Antigen Presenting Bead Kit, HLA-A*02:01

3. Sequencing Kits

Used to amplify, sequence, and analyze differential gene expression profiles.

TCRseq

Used to amplify TCR alpha and beta chains, and prepare them for next-generation sequencing.

Species Available Kits
Human TCRseq Well Plate Kit, Human
RNAseq

Used to amplify and sequence cDNA and analyze differential gene expression profiles.

Species Available Kits
- Single Cell 3' mRNA Kit
Additional Reagents for Your Assays

Viability：Viability stains

Cytotoxicity：Caspase-3 substrate

Cytokine Secretion：Cytokine capture beads、Secondary stains

Surface Phenotype：Surface marker stains

How to Use Your Kit

Supported Cell Types

T cell
CAR-T cell
TCR-T cell
TIL cell
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