High-throughput, automated workflow: Sequentially isolates target single cells from transient co-cultures for downstream analysis, enabling multidimensional characterization of T cell potency at single-cell resolution. Images captured during isolation allow tracking of specific cells or cell types. Applications: Designed for assays requiring co-culture of multiple cell types, it correlates on-chip phenotypic analysis data with single-cell RNA sequencing (scRNA-seq). Unique value: Bridges the gap between existing single-cell functional assessments and parameter correlations by providing interconnected insights into the true biology of individual T cells.